Human Anti-C5 Recombinant Antibody (PABC-462) (CAT#: PABC-462)
This product is a recombinant human antibody that can recognize C5. This antibody bound to the β-chain of C5. This antibody exhibited neutralizing activity to all types of C5 variants tested, suggesting that the activity of the antibody will not be affected by these highly frequent SNPs.
Specific Inquiry
WB
Figure 1 Anti-C5 Recombinant Antibody (PABC-462) in WB
Western blot analysis of PABC-462 was performed with Recombinant Human C5 Protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-462 (2 μg/mL) and HRP Goat Anti-human IgG (H&L) as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
WB
Figure 2 Anti-C5 Recombinant Antibody (PABC-462) in WB
Western blot analysis of PABC-462 was performed with Recombinant Human C5 Protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-462 (2 μg/mL) and HRP Goat Anti-human IgG (H&L) as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Non-Reducing Antigen (0.1 μg)
Lane 2: Non-Reducing Antigen (0.3 μg)
Lane 3: Non-Reducing Antigen (0.6 μg)
DB
Figure 3 Anti-C5 Recombinant Antibody (PABC-462) in DB
Dot blot analysis of PABC-462 was performed by coating with Recombinant Human C5 Protein (His tag). PABC-462 (2 μg/mL) were probed and HRP Goat Anti-human IgG (H&L) as a secondary antibody.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG1, κ
- Species Reactivity
- Human, Cynomolgus monkey
- Applications
- WB, HA, ELISA, FC, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Hemagglutination, Enzyme-linked Immunosorbent Assay, Flow Cytometry and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
Related Resources
Systemic Lupus Erythematosus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "C5"
Select a product category from the dropdown menu below to view related products.
Please select product type
Humanized Antibody Products
Mouse Antibody Products
Neutralizing Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
Human Antibody Products
IgG Antibody Products
ScFv Antibody Products
Single Domain Antibody Products
ADCC Enhanced Antibody Products
Fab Antibody Products
Antibody Magnetic Beads
Hexamer Antibody Products
Non-Human Primate (NHP) Antibody Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-901 | Anti-Human C5 Recombinant Antibody (Pexelizumab) | FuncS, IF, Neut, ELISA, FC, IP, ICC | scFv |
| TAB-1372CL | Human Anti-C5 Recombinant Antibody (TAB-1372CL) | Inhib, FuncS | Humanized antibody |
| TAB-1373CL | Human Anti-C5 Recombinant Antibody (TAB-1373CL) | IB, CDC, Inhib | Humanized IgG |
| TAB-1374CL | Human Anti-C5 Recombinant Antibody (TAB-1374CL) | ELISA, CDC, Inhib | Humanized IgG |
| TAB-1375CL | Human Anti-C5 Recombinant Antibody (TAB-1375CL) | CDC, Inhib | Humanized IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Reliable choice for our research needs
Human Anti-C5 has excellent reproducibility across different experimental conditions, giving us a reliable choice for research.
Excellent
We just introduced Human Anti-C5 Recombinant Antibody to our lab for Western blotting and immunoprecipitation tests, and it has far exceeded our expectations. Its excellent specificity and sensitivity allow it to identify APP and its fragments accurately. This dependability has allowed us to concentrate more on advancing our study, without being distracted by unpredictability or concerns about non-specific interactions.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-462, RRID: AB_3111591)
Copy citation
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
Application: WB, IF, FuncS
-YJ-1.jpg)
Application: ELISA
-1.png)
Application: ELISA
Application: ELISA, FC, Inhib, IHC-Fr, WB, IP
Application: ELISA, Block, WB, FC, IP
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
