Human Anti-C5 Recombinant Antibody (PABC-462)
CAT#: PABC-462
This product is a recombinant human antibody that can recognize C5. This antibody bound to the β-chain of C5. This antibody exhibited neutralizing activity to all types of C5 variants tested, suggesting that the activity of the antibody will not be affected by these highly frequent SNPs.
Tested Data
Gene Expression
WB
Figure 1 Anti-C5 Recombinant Antibody (PABC-462) in WB
Western blot analysis of PABC-462 was performed with Recombinant Human C5 Protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-462 (2 μg/mL) and HRP Goat Anti-human IgG (H&L) as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
WB
Figure 2 Anti-C5 Recombinant Antibody (PABC-462) in WB
Western blot analysis of PABC-462 was performed with Recombinant Human C5 Protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-462 (2 μg/mL) and HRP Goat Anti-human IgG (H&L) as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Non-Reducing Antigen (0.1 μg)
Lane 2: Non-Reducing Antigen (0.3 μg)
Lane 3: Non-Reducing Antigen (0.6 μg)
DB
Figure 3 Anti-C5 Recombinant Antibody (PABC-462) in DB
Dot blot analysis of PABC-462 was performed by coating with Recombinant Human C5 Protein (His tag). PABC-462 (2 μg/mL) were probed and HRP Goat Anti-human IgG (H&L) as a secondary antibody.
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RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (A549, Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000106804-C5
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG1, κ
- Species Reactivity
- Human, Cynomolgus monkey
- Applications
- WB, HA, ELISA, FC, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Hemagglutination, Enzyme-linked Immunosorbent Assay, Flow Cytometry and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
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Reliable choice for our research needs
Human Anti-C5 has excellent reproducibility across different experimental conditions, giving us a reliable choice for research.
Excellent
We just introduced Human Anti-C5 Recombinant Antibody to our lab for Western blotting and immunoprecipitation tests, and it has far exceeded our expectations. Its excellent specificity and sensitivity allow it to identify APP and its fragments accurately. This dependability has allowed us to concentrate more on advancing our study, without being distracted by unpredictability or concerns about non-specific interactions.
Q&As
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What is the optimal dilution for using SKY59 in WB?
A: The optimal dilution for using the Human Anti-C5 Recombinant Antibody (PABC-462) in immunofluorescence is typically 1:1000 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for specific experimental conditions.
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When can I expect to receive the antibodies after placing the order?
A: You can ask customer service for details.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-462, RRID: AB_3111591)
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Related Diseases
Systemic Lupus Erythematosus
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-462 | Human Anti-C5 Recombinant Antibody Fab Fragment (PFBC-462) | ELISA, WB, FC, Neut | Human Fab |
| HPAB-M0263-YC-F(E) | Human Anti-C5 Recombinant Antibody (clone Antibody A); Fab Fragment | Inhib, FuncS | Human Fab |
| HPAB-M0264-YC-F(E) | Human Anti-C5 Recombinant Antibody (clone Antibody B); Fab Fragment | Inhib, FuncS | Human Fab |
| HPAB-1484-FY-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-1484-FY-S(P)) | ELISA, Neut | Mouse scFv |
| HPAB-1485-FY-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-1485-FY-S(P)) | ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-453CQ | Afuco™ Anti-C5 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-453CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-008 | Afuco™ Anti-C5 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-008) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-436CQ | Afuco™ Anti-C5 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-436CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1029-FY | Human Anti-C5 Recombinant Antibody (HPAB-1029-FY) | ELISA | Human IgG1 |
| HPAB-1031-FY | Human Anti-C5 Recombinant Antibody (HPAB-1031-FY) | ELISA | Human IgG1 |
| HPAB-1032-FY | Human Anti-C5 Recombinant Antibody (HPAB-1032-FY) | ELISA | Human IgG1 |
| HPAB-1033-FY | Human Anti-C5 Recombinant Antibody (HPAB-1033-FY) | ELISA | Human IgG1 |
| HPAB-1034-FY | Human Anti-C5 Recombinant Antibody (HPAB-1034-FY) | ELISA | Human IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0924-YC104 | Mouse Anti-C5 Recombinant Antibody Hexamer (VS-0924-YC104), CDC Enhanced | ELISA, Inhib, CDC | Antibody hexamer |
| CAT | Product Name | Application | Type |
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| VS-1024-XY137 | Mouse Anti-NHP C5 Recombinant Antibody (clone aE11) | ELISA | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY95 | Human Anti-C5 (clone BB5.1) scFv-Fc Chimera | FC, IA, IF, IP, IHC, FuncS | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0525-XY889 | Anti-C5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY890 | Anti-Rat C5 Immunohistochemistry Kit | IHC |
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